Application of Yingtai Centrifugal Concentrators in DNA/RNA Concentration

Application of Yingtai Centrifugal Concentrators in DNA/RNA Concentration  

Centrifugal concentrators demonstrate significant technical advantages in DNA/RNA concentration and have become a critical tool in molecular biology research. By combining vacuum pressure (50-100 mbar) to reduce solvent boiling points, gentle heating (30-40°C), and centrifugal force (1000-1500 rpm), this equipment can efficiently concentrate trace nucleic acid samples (50μL → 5μL) within 30-60 minutes while maintaining DNA/RNA integrity (recovery rate ≥95%). Its closed-system design effectively prevents aerosol contamination and supports high-throughput 96-well plate processing, outperforming traditional ethanol precipitation methods (saving 60% time and avoiding salt residue).  

Typical applications include:  

- NGS library preparation  

- PCR product purification  

- Handling precious samples (e.g., cDNA concentration in single-cell transcriptome sequencing).  

Some models also feature integrated condenser traps for safe solvent recovery, complying with GLP laboratory standards.  

1. Advantages of Centrifugal Concentrators  

Centrifugal concentrators, with their gentle yet efficient performance, have become essential for nucleic acid (DNA/RNA) concentration and purification in molecular biology. Key advantages include:  

1.1 Sample Integrity Protection  

- Low-temperature (30-40°C) vacuum concentration prevents nucleic acid denaturation (e.g., DNA fragmentation or RNA degradation).  

- Recovery rates improve by ≥20% compared to traditional ethanol precipitation (literature data).  

1.2 High-Throughput Processing  

- Simultaneously processes 48-180 microsamples (compatible with 1.5 mL tubes).  

- Automated operation reduces manual handling time by 60%.  

1.3 Broad Compatibility  

- Works with various extraction buffers (TE buffer, Tris-HCl) and organic solvents (isopropanol, ethanol).  

- Concentrates pg to μg levels of nucleic acids, meeting downstream needs (e.g., NGS, PCR).  

2. Yingtai Vacuum Centrifugal Concentrator  

The Yingtai Vacuum Centrifugal Concentrator enables parallel processing of multiple samples without cross-contamination risks. Key features include:  

- Programmable settings (up to 30 protocols).  

- Real-time sample imaging to monitor concentration progress.  

- Adjustable vacuum control (Pirani vacuum gauge ensures accuracy).  

- Customizable centrifugation speeds for different sample types.  

This system ensures High efficiency, precision, and compliance with advanced laboratory standards.