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Technical Document: Factors Related To Sedimentation Separation in Yingtai Floor-Standing High-Capacity Refrigerated Centrifuges

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Technical Document: Factors Related To Sedimentation Separation in Yingtai Floor-Standing High-Capacity Refrigerated Centrifuges


Factors related to sedimentation separation in floor-standing high-capacity refrigerated centrifuges include centrifugal speed, centrifugal radius, centrifugal temperature, and centrifugal time.


1. Centrifugal Speed:

  - The speed of centrifugation determines the sedimentation rate of particles. Different sizes of particles require different centrifugal speeds. Larger particles, which have greater mass, settle faster in the centrifugal field and can be centrifuged at lower speeds. Conversely, smaller particles require higher speeds for effective separation.


2. Centrifugal Radius:

  - The set centrifugal radius defines the capacity of the centrifuge.

  - The centrifugal radius determines the sedimentation distance of the particles. During gradient centrifugation, a shorter sedimentation distance makes it more challenging to separate substances.


3. Centrifugal Temperature:

  - The viscosity of different media varies with temperature. Most centrifugal media change viscosity with temperature variations. Therefore, maintaining a constant temperature during centrifugation is essential. Gradient centrifugation is particularly sensitive to temperature, requiring stricter control of the centrifugal temperature.


4. Centrifugal Time:

  - If the centrifugation time is shorter than the clarification time, not all target particles can be obtained.

  - If the centrifugation time exceeds the clarification time, it not only wastes time and equipment but may also increase the presence of non-target particles.



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