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Types of Yingtai Centrifuge Rotors
Common Types of Centrifuge Rotors
Angle Rotor
In a fixed-angle rotor, the angle between the centrifuge tube and the rotor's axis is set at a specific angle, typically ranging from 14° to 45°.
- This type of rotor has a low center of gravity, long lifespan, large capacity, and can achieve high rotational speeds, with a maximum centrifugal force of up to 800,000 g.
- It is mainly used for separating samples with significantly different sedimentation velocities.
- Particles in a fan-shaped solution move a short distance and, upon contacting the outer wall, slide down along the wall to form sediment at the bottom. Therefore, this rotor can quickly collect sediments.
- However, the interaction with the tube wall during centrifugation induces strong convection, which can negatively affect particles with similar sedimentation rates.
- Fixed-angle rotors are standard configurations in most laboratories and can meet the needs of a wide range of experiments.
Swing Rotor
Also known as a swinging bucket rotor, in a Swing rotor, the centrifuge tubes are parallel to the rotor axis when stationary. As the rotor accelerates, the tube's axis transitions from parallel to perpendicular (90°) to the rotor axis.
- This type of rotor is primarily used for density gradient centrifugation. Particles travel longer distances, and the corresponding centrifugation time is generally longer, allowing the components in the solution to remain positioned relative to the tube wall throughout the centrifugation process, thus enhancing the separation effectiveness.
- However, as particles disperse radially from the rotation rather than settling in parallel paths, they still slide down the wall upon contact, which can also create convection, albeit less than in fixed-angle rotors.
- Low-speed starts or stops may produce vibrations, affecting the separation results.
- Horizontal rotors are further divided into open and closed types. Open rotors, typically with larger preparative capacities, have speeds below 10,000 rpm and centrifugal forces within 16,000 x g, mainly used for initial sample separation. Closed rotors, with smaller preparative capacities and speeds above 10,000 rpm, operate at centrifugal forces above 16,000 x g to minimize air resistance, primarily used for the separation of organelles such as mitochondria, chloroplasts, and nuclei, as well as for density gradient centrifugation. Additionally, rotors for centrifuging microtiter plates can also be categorized as horizontal rotors.
We also have PRP Rotor, Microplate Rotor, Milk testing Rotor, Vertical Rotor....