Yingtai: Applications of Microcentrifuge Column Technology

Yingtai: Applications of Microcentrifuge Column Technology

I. Molecular Biology and Genetic Research  

- High-Throughput Nucleic Acid Extraction for Genomic Sequencing Library Construction  

  Applications: Whole genome sequencing, single-cell sequencing; DNA fragment purification after CRISPR gene editing; large-scale epidemic screening (e.g., batch RNA extraction for COVID-19, influenza viruses).  

  Scenarios: Compatible with 96-well or 384-well plates, enabling simultaneous processing of dozens to hundreds of samples per centrifugation.  

  Advantages: Reduces manual operation errors and improves throughput efficiency (extraction time can be shortened to 15 minutes per batch).  

- Microsample Processing  

  Applications: Single-cell RNA extraction (combined with microfluidic technology for adsorbing trace RNA); ancient DNA research (enriching degraded DNA fragments from fossils or archaeological samples); liquid biopsy (capturing circulating tumor DNA from 1 mL of plasma).  

  Innovative Design: Nanocoated centrifuge columns enhance low-concentration nucleic acid adsorption rates (e.g., silica-based magnetic bead modification technology).  

II. Clinical Diagnostics and Precision Medicine  

- Rapid Pathogen Detection  

  Applications: Nucleic acid enrichment for respiratory viruses (e.g., influenza A, respiratory syncytial virus); DNA/RNA separation for blood pathogens (e.g., HIV, malaria parasites); genomic extraction of gut microbiota from fecal samples (for diagnosing microbial dysbiosis).  

  Technical Integration: Combined with qPCR or nanopore sequencers to achieve "extraction-detection" integration.  

- Tumor Molecular Profiling  

  Applications: Purification of trace DNA from FFPE (formalin-fixed paraffin-embedded) tissues for driver gene detection (e.g., EGFR, KRAS mutations); enrichment of methylated DNA (selective adsorption of specific modified fragments via centrifuge columns).  

  Clinical Value: Assists in targeted therapy and prognosis evaluation, with sensitivity reaching 0.1% mutation frequency.  

III. Industrial and Synthetic Biology  

- Recombinant Protein Purification  

  Applications: Removal of host proteins and endotoxins from E. coli expression systems; purification of antibody drugs secreted by mammalian cells (e.g., IgG binding to Protein A/G columns).  

  Parameter Optimization: Gradient centrifugation (500–3,000×g) for separating impurities of different molecular weights.  

- Synthetic Biology Component Preparation  

  Applications: Plasmid DNA purification (for gene delivery vector synthesis); siRNA/mRNA purification (removing enzymes and nucleotide residues from in vitro transcription reactions); precise recovery of DNA fragments in artificial chromosome construction.  

  Trend: Automated synthesis platforms integrated with centrifuge columns, supporting a "design-build-purify" closed-loop process.  

IV. Environmental and Food Safety Monitoring  

- Environmental Microbe Detection  

  Applications: Nucleic acid enrichment for pathogens in water/soil (e.g., Legionella, Salmonella); concentrated detection of viral aerosols in air samples (e.g., avian influenza virus monitoring).  

  Technical Improvement: Inhibitor-resistant centrifuge columns (with PEG modification) enhance extraction efficiency from complex environmental samples.  

- Food Contaminant Screening  

  Applications: Detection of genetically modified components (extracting plant DNA from processed foods); identification of foodborne pathogens (e.g., Listeria DNA extraction from meat products); allergen tracing (separating protein residues such as peanuts and gluten via centrifuge columns).  

  Regulatory Compliance: Meets ISO 24276 and other standards, supporting high-throughput compliance testing.