​Yingtai: Under What Circumstances Might Difficulties Arise When Using High-power Objectives?

Yingtai: Under what circumstances might difficulties arise when using high-power objectives?

When using a microscope, difficulties may arise when using high-power objectives in the following situations:

1. Uneven Sample Thickness: If the sample surface is not flat or has height variations, it can be challenging to keep all parts within the focal range when using high-power objectives, as they have a very shallow depth of field.

2. Sample Size Too Large: If the sample exceeds the effective viewing range, high-power objectives may not be able to clearly observe the entire sample because of their short working distance, which might not cover the whole sample.

3. Insufficient Sample Transparency: If the sample is opaque or has strong scattering properties, the imaging may be severely affected when using high-power objectives, as they require high light quality.

4. Insufficient Light Source Brightness: If the illumination brightness is not adequate, it may be impossible to provide enough light to illuminate the sample when using high-power objectives, thus affecting observation quality.

5. Insufficient User Skill: For beginners, achieving precise focusing and handling when using high-power objectives can be difficult, as they require high operational precision.

Therefore, before using high-power objectives, it's advisable to assess the properties of the sample and experimental needs. If necessary, perform preliminary observation and focusing with lower-power objectives to better prepare the sample and adjust the microscope settings, ensuring a higher success rate in observations with high-power objectives.